Enzymatic synthesis of RNA oligonucleotides

Enzymatic synthesis of RNA oligonucleotides.

Using SP6 RNA polymerase and various synthetic DNA oligomers as templates (12-65 b), we have obtained efficient synthesis of RNA transcripts without the need for either primer or promoter. Gel analysis of transcripts made from templates of known size demonstrated a predominant single species, together with some minor bands. For a given template the major band had the same 5'-nucleotide as predi...

Enzymatic synthesis of 20-methylseleno-modified RNA†

Selenium-derivatization of RNA is a powerful and advantageous alternative to conventional heavy atom derivatization techniques that are required for the phasing of X-ray crystallographic diffraction data. Among several possibilities, the 20-methylseleno (20-SeCH3) modification has been most widely explored and was responsible for a series of important RNA structure determinations, such as the D...

Enzymatic RNA Synthesis using Bacteriophage T7 RNA Polymerase

Replication of Viral Rna. Iv. Properties of Rna Synthetase and Enzymatic Synthesis of Ms2 Phage Rna.

Evidence has been presented' for the accumulation of a double-stranded replicative form of MS2 RNA in vivo, following infection of Escherichia coli with MS2 phage, and for the identity of this material with the double-stranded RNA which is labeled in vitro with radioactive carbon2 on incubation of CL4-labeled ribonucleoside triphosphates with RNA synthetase.3 The present paper is concerned with...

In Silico Design and Enzymatic Synthesis of Functional RNA Nanoparticles

CONSPECTUS: The use of RNAs as scaffolds for biomedical applications has several advantages compared with other existing nanomaterials. These include (i) programmability, (ii) precise control over folding and self-assembly, (iii) natural functionalities as exemplified by ribozymes, riboswitches, RNAi, editing, splicing, and inherent translation and transcription control mechanisms, (iv) biocomp...